Precision at 260 nm: Cutting-Edge Strategies for Nucleic Acid Measurement
Accurate measurement of nucleic acids underpins everything from cloning and qPCR to transcriptomics and next-generation sequencing. The tools are powerful, the stakes are high, and the margins for error can be razor thin. Mastery of DNA and RNA quantification hinges on understanding absorbance fundamentals, recognizing contaminants, and selecting instrumentation that matches sample volume, throughput, and